FOX04 – 10mg

FOXO4-DRI (D-Retro-Inverso)—often referred to simply as the FOXO4 peptide or Proxofim—is a cell-permeable, synthetic senolytic peptide designed to target and eliminate senescent cells. Senescent cells, or “zombie cells,” resist natural cell death by overexpressing the FOXO4 transcription factor, which sequesters the p53 tumor suppressor protein inside the nucleus. Formulated with mirror-image D-amino acids in a reversed sequence (retro-inverso modification), FOXO4-DRI features extraordinary resistance to enzymatic degradation, enabling prolonged experimental stability in complex physiological models. [1, 2, 3]

Key Features & Characteristics

• Mechanism of Action: Competitively disrupts the protein-protein interaction between FOXO4 and p53, triggering p53 nuclear exclusion and directing it to the mitochondria to induce selective apoptosis. [1, 2]
• Targeted Specificity: Purposely clears damaged, non-dividing senescent cells while leaving healthy, proliferating surrounding cells unharmed. [1, 2]
• Enhanced Stability: The D-retro-inverso configuration prevents rapid cellular protease digestion, offering an exceptionally extended half-life during in vitro and in vivo studies. [1]
• Physical Form: Synthesized to strict purity standards and supplied as a highly stable, white lyophilized powder ready for precise laboratory reconstitution. [1, 2]

Primary Research Applications

• Cellular Rejuvenation & Longevity: Heavily studied in mouse and tissue models to analyze the reversal of age-related phenotypes, including the restoration of fur density, kidney performance, and physical activity markers.
• Endocrine & Leydig Cell Dysfunction: Evaluated for its potential to clear senescent testicular cells, improve the microenvironment, and counter age-related testosterone secretion deficits.
• Vascular & Tissue Aging: Investigated for its impact on suppressing aortic aging by clearing senescent endothelial cells, thereby helping to reduce systemic inflammation and evaluate arterial flexibility. [1, 2, 4, 5]

For Research Use Only. Not for human consumption, therapeutic, or diagnostic use.